Light microscopy makes it possible to see structures up to a size of about 300 nanometers. This means that practically all pro- and eukaryotic cells as well as the organelles can be observed within the cells and their dynamics can be analyzed. For smaller structures, on the other hand, the electron microscope is usually required, since the resolution of the light microscope is limited by the quality of the lenses (numerical aperture) and the wavelength of the microscopy light. [more]
